af4 instrument af2000 Search Results


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Postnova Analytics af4 instrument
Af4 Instrument, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Waters Corporation eclipse af4
Fig. 2. <t>MD-AF4</t> results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Eclipse Af4, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse af4/product/Waters Corporation
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Postnova Analytics mwco value
Fig. 2. <t>MD-AF4</t> results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Mwco Value, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Postnova Analytics af4 system
Fig. 2. <t>MD-AF4</t> results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Af4 System, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Waters Corporation dawn
Fig. 2. <t>MD-AF4</t> results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Dawn, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Postnova Analytics spd 20a uv vis spectrophotometer
Fig. 2. <t>MD-AF4</t> results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Spd 20a Uv Vis Spectrophotometer, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Postnova Analytics cellulose membrane
Fig. 2. <t>MD-AF4</t> results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Cellulose Membrane, supplied by Postnova Analytics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Waters Corporation refractive index
Fig. 2. <t>MD-AF4</t> results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Refractive Index, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Laserchrom HPLC Laboratories uv/vis s3210
Fig. 2. <t>MD-AF4</t> results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.
Uv/Vis S3210, supplied by Laserchrom HPLC Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 2. MD-AF4 results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery.

doi: 10.1016/j.ejpb.2021.03.004

Figure Lengend Snippet: Fig. 2. MD-AF4 results using the LC. A) UV fractograms of LNP1-RNA overlaid with Rg values, measured by Lab1 using the LC with two different XF profiles (linear and exponential) presented in figure C, showing (i) a large void peak (orange arrow), (ii) the main peak associated with NPs (violet arrow) and (iii) a large, retained peak (light blue arrow). ; B) UV fractograms of the same sample overlaid with Rg values, measured by Lab2 using the LC with the linearly decreasing XF profile of Figure C but with focusing times of 4, 7 and 9 min. C) XF profiles applied: exponential (black) and linear (red) decreases of the XF. D) Void peak height as % of the main peak for varying focus times with linear XF profile, Lab2. E) Retained peak height as % of the main peak for linear and exponential XF profiles, Lab1.

Article Snippet: Two widely available commercial platforms were used for this study: in Lab1, an AF2000 Multiflow FFF (Postnova Analytics) and in Lab2 an Eclipse AF4 (Wyatt Technology).

Techniques:

Fig. 3. Comparison of the FI and LC AF4 methods. Fractograms obtained for LNP1-RNA by applying a linearly decreasing XF profile for Lab1 (black) and Lab 2 (red) using (A) the LC and (B) the FI channel. Light scattering intensity and UV–Vis absorption are reported on the left and right panels, respectively, together with the Rg profile estimated by Berry model. C) Summary of the results averaged over 3 measurements. Values in parentheses represent the standard deviation of the mean.

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery.

doi: 10.1016/j.ejpb.2021.03.004

Figure Lengend Snippet: Fig. 3. Comparison of the FI and LC AF4 methods. Fractograms obtained for LNP1-RNA by applying a linearly decreasing XF profile for Lab1 (black) and Lab 2 (red) using (A) the LC and (B) the FI channel. Light scattering intensity and UV–Vis absorption are reported on the left and right panels, respectively, together with the Rg profile estimated by Berry model. C) Summary of the results averaged over 3 measurements. Values in parentheses represent the standard deviation of the mean.

Article Snippet: Two widely available commercial platforms were used for this study: in Lab1, an AF2000 Multiflow FFF (Postnova Analytics) and in Lab2 an Eclipse AF4 (Wyatt Technology).

Techniques: Comparison, Standard Deviation

Fig. 7. LNP1-RNA particle morphology by MD-AF4. A) Rg and Rh profiles of LNP1-RNA measured by online DLS and online MALS; B) calculated Rg/Rh ratio across the peak.

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: Improved multidetector asymmetrical-flow field-flow fractionation method for particle sizing and concentration measurements of lipid-based nanocarriers for RNA delivery.

doi: 10.1016/j.ejpb.2021.03.004

Figure Lengend Snippet: Fig. 7. LNP1-RNA particle morphology by MD-AF4. A) Rg and Rh profiles of LNP1-RNA measured by online DLS and online MALS; B) calculated Rg/Rh ratio across the peak.

Article Snippet: Two widely available commercial platforms were used for this study: in Lab1, an AF2000 Multiflow FFF (Postnova Analytics) and in Lab2 an Eclipse AF4 (Wyatt Technology).

Techniques: